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We recently reported that p28, one of the two turnip crinkle virus (TCV) replication proteins, trans-complemented a defective TCV lacking p28, yet repressed the replication of another TCV replicon encoding wildtype p28 (Zhang et al., 2017). Here we show that p88, the TCV-encoded RNA-dependent RNA polymerase, likewise trans-complemented a p88-defective TCV replicon, but repressed one encoding wild-type p88. Surprisingly, lowering p88 protein levels enhanced trans-complementation, but weakened repression. Repression by p88 was not simply due to protein over-expression, as deletion mutants missing 127 or 224 N-terminal amino acids accumulated to higher levels but were poor repressors. Finally, both trans-complementation and repression by p88 were accompanied by preferential accumulation of subgenomic RNA2, and a novel class of small TCV RNAs. Our results suggest that repression of TCV replication by p88 may manifest a viral mechanism that regulates the ratio of genomic and subgenomic RNAs based on p88 abundance. 

We recently reported that p28, one of the two turnip crinkle virus (TCV) replication proteins, trans-complemented a defective TCV lacking p28, yet repressed the replication of another TCV replicon encoding wildtype p28 (Zhang et al., 2017). Here we show that p88, the TCV-encoded RNA-dependent RNA polymerase, likewise trans-complemented a p88-defective TCV replicon, but repressed one encoding wild-type p88. Surprisingly, lowering p88 protein levels enhanced trans-complementation, but weakened repression. Repression by p88 was not simply due to protein over-expression, as deletion mutants missing 127 or 224 N-terminal amino acids accumulated to higher levels but were poor repressors. Finally, both trans-complementation and repression by p88 were accompanied by preferential accumulation of subgenomic RNA2, and a novel class of small TCV RNAs. Our results suggest that repression of TCV replication by p88 may manifest a viral mechanism that regulates the ratio of genomic and subgenomic RNAs based on p88 abundance. 

How bio-membranes are self-organized to perform their functions remains a pivotal issue in biological and chemical science. Understanding the self-assembly principles of lipid-like molecules hence becomes crucial. Here we report the meso-structural evolution of amphiphilic sphere-rod conjugates (giant lipids), and study the roles of geometric parameters (head-tail ratio and cross-section area) during this course. As a prototype system, giant lipids resemble natural lipidic molecules by capturing their essential features including head-tail configuration, monodispersed molecular weight distribution and minor interpenetration of hydrophobic tails. We demonstrate the self-assembly behavior of two categories of giant lipids (I-shape and T-shape, a total of 8 molecules). A rich variety of meso-structures are constructed in solution state and their molecular packing models are rationally understood. We streamline the driving forces of morphological evolution from both geometric and thermodynamic perspective. Giant lipids recast the phase behavior of both linear and branched lipidic molecules to certain degree, while the abundant self-assembled morphologies reveal distinct physiochemical behaviors when geometric parameters deviate from natural analogues. 

A<sc>bstract</sc> Inclusive and differential cross sections for Higgs boson production in proton-proton collisions at a centre-of-mass energy of 13.6 TeV are measured using data collected with the CMS detector at the LHC in 2022, corresponding to an integrated luminosity of 34.7 fb⁻¹. Events with the diphoton final state are selected, and the measured inclusive fiducial cross section is$${\sigma }_{\text{fid}}={74}\pm {11}{\left({\text{stat}}\right)}_{-4}^{+5}\left({\text{syst}}\right)$$fb, in agreement with the standard model prediction of 67.8 ± 3.8 fb. Differential cross sections are measured as functions of several observables: the Higgs boson transverse momentum and rapidity, the number of associated jets, and the transverse momentum of the leading jet in the event. Within the uncertainties, the differential cross sections agree with the standard model predictions. 

A measurement is presented of the cross section in proton-proton collisions for the production of two $W$bosons and one $Z$boson. It is based on data recorded by the CMS experiment at the CERN LHC at center-of-mass energies $\sqrt{s}=13$and 13.6 TeV, corresponding to an integrated luminosity of $200{\mathrm{fb}}^{-1}$. Events with four charged leptons (electrons or muons) in the final state are selected. Both nonresonant $WWZ$production and $ZH$production, with the Higgs boson decaying into two $W$bosons, are reported. For the first time, the two processes are measured separately in a simultaneous fit. Combining the two modes, signal strengths relative to the standard model (SM) predictions of ${0.75}_{-0.29}^{+0.34}$and ${1.74}_{-0.60}^{+0.71}$are measured for $\sqrt{s}=13$and 13.6 TeV, respectively. The observed (expected) significance for the triboson signal is 3.8 (2.5) standard deviations for $\sqrt{s}=13.6\mathrm{TeV}$, thus providing the first evidence for triboson production at this center-of-mass energy. Combining the two modes and the two center-of-mass energies, the inclusive signal strength relative to the SM prediction is measured to be ${1.03}_{-0.28}^{+0.31}$, with an observed (expected) significance of 4.5 (5.0) standard deviations. 

A<sc>bstract</sc> The first measurement of the dijet transverse momentum balancex_jin proton-lead (pPb) collisions at a nucleon-nucleon center-of-mass energy of$$\sqrt{{s}_{\text{NN}}}$$= 8.16 TeV is presented. Thex_jobservable, defined as the ratio of the subleading over leading jet transverse momentum in a dijet pair, is used to search for jet quenching effects. The data, corresponding to an integrated luminosity of 174.6 nb⁻¹, were collected with the CMS detector in 2016. Thex_jdistributions and their average values are studied as functions of the charged-particle multiplicity of the events and for various dijet rapidity selections. The latter enables probing hard scattering of partons carrying distinct nucleon momentum fractionsxin the proton- and lead-going directions. The former, aided by the high-multiplicity triggers, allows probing for potential jet quenching effects in high-multiplicity events (with up to 400 charged particles), for which collective phenomena consistent with quark-gluon plasma (QGP) droplet formation were previously observed. The ratios ofx_jdistributions for high- to low-multiplicity events are used to quantify the possible medium effects. These ratios are consistent with simulations of the hard-scattering process that do not include QGP production. These measurements set an upper limit on medium-induced energy loss of the subleading jet of 1.26% of its transverse momentum at the 90% confidence level in high multiplicity pPb events. 

A search is presented for fractionally charged particles with charges below $1e$, using their small energy loss in the tracking detector as a key variable to observe a signal. The analyzed dataset corresponds to an integrated luminosity of $138{\mathrm{fb}}^{-1}$of proton-proton collisions collected at $\sqrt{s}=13\mathrm{TeV}$in 2016–2018 at the CERN LHC. This is the first search at the LHC for new particles with a charge between $e/3$and $0.9e$, including an extension of previous results at a charge of $2e/3$. Masses up to 640 GeV and charges as low as $e/3$are excluded at 95% confidence level. These are the most stringent limits to date for the considered Drell-Yan-like production mode.